ibm 192 statistics 22 windows Search Results


22 k human oligo array version 1.0  (CapitalBio Corporation)
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rare t1 sequence  (Bruker Corporation)
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Bruker Corporation rare t1 sequence
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trio 3t scanner  (Siemens AG)
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balb c female mice  (Pasteur Institute)
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mirna mimics mir-15a  (Shanghai GenePharma)
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Identification of candidate miRNAs targeting lncRNA PCAT-1 in HCC. A and B, miR-215 inhibits PCAT-1 expression. 20 nmol/liter mimics of <t>miR-15a,</t> miR-16, miR-22, <t>miR-93a,</t> miR-105, <t>miR-106a,</t> miR-129, miR-138, <t>miR-181a,</t> miR-192, miR-205, miR-215, <t>miR-291a,</t> miR-294, miR-295, miR-302, miR-372, miR-373, miR-383, and miR-428, siRNA duplexes (siPCAT-1-1, siPCAT-1-2) or NC RNA were transfected into SMMC7721 and Huh7 cells. lncRNA PCAT-1 expression was detected at 48 h after transfection (left, black columns). The transfection efficiency of different miRNAs was confirmed by qRT-PCR (right, gray columns). C and D, inhibition of miR-215 up-regulates PCAT-1 expression. 20 nmol/liter of miR-215 inhibitors and NC RNA were transfected into SMMC7721 and Huh7 HCC cells. All data of PCAT-1 expression were normalized to β-actin mRNA expression levels. All <t>miRNA</t> expression data were normalized to U6 small RNA expression. E, schematic constructions of pGL3-PCAT-1 and pGL3-Mut215. F, pGL3-PCAT-1 and pGL3-Mut215 were co-transfected into SMMC7721 and Huh7 cells with miR-215 mimics or NC RNA. Luciferase activity was detected at 48 h after transfection and normalized relative to the Renilla luciferase expression. Inhibition effects of miR-215 mimics on pGL3-PCAT-1 or pGL3-Mut215 are shown. All results of the mean of triplicate assays with standard deviation are presented. *, p < 0.05; **, p < 0.01.
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fluorescein isothiocyanate  (Thermo Fisher)
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Thermo Fisher fluorescein isothiocyanate
Identification of candidate miRNAs targeting lncRNA PCAT-1 in HCC. A and B, miR-215 inhibits PCAT-1 expression. 20 nmol/liter mimics of <t>miR-15a,</t> miR-16, miR-22, <t>miR-93a,</t> miR-105, <t>miR-106a,</t> miR-129, miR-138, <t>miR-181a,</t> miR-192, miR-205, miR-215, <t>miR-291a,</t> miR-294, miR-295, miR-302, miR-372, miR-373, miR-383, and miR-428, siRNA duplexes (siPCAT-1-1, siPCAT-1-2) or NC RNA were transfected into SMMC7721 and Huh7 cells. lncRNA PCAT-1 expression was detected at 48 h after transfection (left, black columns). The transfection efficiency of different miRNAs was confirmed by qRT-PCR (right, gray columns). C and D, inhibition of miR-215 up-regulates PCAT-1 expression. 20 nmol/liter of miR-215 inhibitors and NC RNA were transfected into SMMC7721 and Huh7 HCC cells. All data of PCAT-1 expression were normalized to β-actin mRNA expression levels. All <t>miRNA</t> expression data were normalized to U6 small RNA expression. E, schematic constructions of pGL3-PCAT-1 and pGL3-Mut215. F, pGL3-PCAT-1 and pGL3-Mut215 were co-transfected into SMMC7721 and Huh7 cells with miR-215 mimics or NC RNA. Luciferase activity was detected at 48 h after transfection and normalized relative to the Renilla luciferase expression. Inhibition effects of miR-215 mimics on pGL3-PCAT-1 or pGL3-Mut215 are shown. All results of the mean of triplicate assays with standard deviation are presented. *, p < 0.05; **, p < 0.01.
Fluorescein Isothiocyanate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cheminform  (Verlag GmbH)
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Verlag GmbH cheminform
Identification of candidate miRNAs targeting lncRNA PCAT-1 in HCC. A and B, miR-215 inhibits PCAT-1 expression. 20 nmol/liter mimics of <t>miR-15a,</t> miR-16, miR-22, <t>miR-93a,</t> miR-105, <t>miR-106a,</t> miR-129, miR-138, <t>miR-181a,</t> miR-192, miR-205, miR-215, <t>miR-291a,</t> miR-294, miR-295, miR-302, miR-372, miR-373, miR-383, and miR-428, siRNA duplexes (siPCAT-1-1, siPCAT-1-2) or NC RNA were transfected into SMMC7721 and Huh7 cells. lncRNA PCAT-1 expression was detected at 48 h after transfection (left, black columns). The transfection efficiency of different miRNAs was confirmed by qRT-PCR (right, gray columns). C and D, inhibition of miR-215 up-regulates PCAT-1 expression. 20 nmol/liter of miR-215 inhibitors and NC RNA were transfected into SMMC7721 and Huh7 HCC cells. All data of PCAT-1 expression were normalized to β-actin mRNA expression levels. All <t>miRNA</t> expression data were normalized to U6 small RNA expression. E, schematic constructions of pGL3-PCAT-1 and pGL3-Mut215. F, pGL3-PCAT-1 and pGL3-Mut215 were co-transfected into SMMC7721 and Huh7 cells with miR-215 mimics or NC RNA. Luciferase activity was detected at 48 h after transfection and normalized relative to the Renilla luciferase expression. Inhibition effects of miR-215 mimics on pGL3-PCAT-1 or pGL3-Mut215 are shown. All results of the mean of triplicate assays with standard deviation are presented. *, p < 0.05; **, p < 0.01.
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s aureus atcc  (ATCC)
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ATCC s aureus atcc
Identification of candidate miRNAs targeting lncRNA PCAT-1 in HCC. A and B, miR-215 inhibits PCAT-1 expression. 20 nmol/liter mimics of <t>miR-15a,</t> miR-16, miR-22, <t>miR-93a,</t> miR-105, <t>miR-106a,</t> miR-129, miR-138, <t>miR-181a,</t> miR-192, miR-205, miR-215, <t>miR-291a,</t> miR-294, miR-295, miR-302, miR-372, miR-373, miR-383, and miR-428, siRNA duplexes (siPCAT-1-1, siPCAT-1-2) or NC RNA were transfected into SMMC7721 and Huh7 cells. lncRNA PCAT-1 expression was detected at 48 h after transfection (left, black columns). The transfection efficiency of different miRNAs was confirmed by qRT-PCR (right, gray columns). C and D, inhibition of miR-215 up-regulates PCAT-1 expression. 20 nmol/liter of miR-215 inhibitors and NC RNA were transfected into SMMC7721 and Huh7 HCC cells. All data of PCAT-1 expression were normalized to β-actin mRNA expression levels. All <t>miRNA</t> expression data were normalized to U6 small RNA expression. E, schematic constructions of pGL3-PCAT-1 and pGL3-Mut215. F, pGL3-PCAT-1 and pGL3-Mut215 were co-transfected into SMMC7721 and Huh7 cells with miR-215 mimics or NC RNA. Luciferase activity was detected at 48 h after transfection and normalized relative to the Renilla luciferase expression. Inhibition effects of miR-215 mimics on pGL3-PCAT-1 or pGL3-Mut215 are shown. All results of the mean of triplicate assays with standard deviation are presented. *, p < 0.05; **, p < 0.01.
S Aureus Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Identification of candidate miRNAs targeting lncRNA PCAT-1 in HCC. A and B, miR-215 inhibits PCAT-1 expression. 20 nmol/liter mimics of miR-15a, miR-16, miR-22, miR-93a, miR-105, miR-106a, miR-129, miR-138, miR-181a, miR-192, miR-205, miR-215, miR-291a, miR-294, miR-295, miR-302, miR-372, miR-373, miR-383, and miR-428, siRNA duplexes (siPCAT-1-1, siPCAT-1-2) or NC RNA were transfected into SMMC7721 and Huh7 cells. lncRNA PCAT-1 expression was detected at 48 h after transfection (left, black columns). The transfection efficiency of different miRNAs was confirmed by qRT-PCR (right, gray columns). C and D, inhibition of miR-215 up-regulates PCAT-1 expression. 20 nmol/liter of miR-215 inhibitors and NC RNA were transfected into SMMC7721 and Huh7 HCC cells. All data of PCAT-1 expression were normalized to β-actin mRNA expression levels. All miRNA expression data were normalized to U6 small RNA expression. E, schematic constructions of pGL3-PCAT-1 and pGL3-Mut215. F, pGL3-PCAT-1 and pGL3-Mut215 were co-transfected into SMMC7721 and Huh7 cells with miR-215 mimics or NC RNA. Luciferase activity was detected at 48 h after transfection and normalized relative to the Renilla luciferase expression. Inhibition effects of miR-215 mimics on pGL3-PCAT-1 or pGL3-Mut215 are shown. All results of the mean of triplicate assays with standard deviation are presented. *, p < 0.05; **, p < 0.01.

Journal: The Journal of Biological Chemistry

Article Title: The long noncoding RNA PCAT-1 links the microRNA miR-215 to oncogene CRKL-mediated signaling in hepatocellular carcinoma

doi: 10.1074/jbc.M116.773978

Figure Lengend Snippet: Identification of candidate miRNAs targeting lncRNA PCAT-1 in HCC. A and B, miR-215 inhibits PCAT-1 expression. 20 nmol/liter mimics of miR-15a, miR-16, miR-22, miR-93a, miR-105, miR-106a, miR-129, miR-138, miR-181a, miR-192, miR-205, miR-215, miR-291a, miR-294, miR-295, miR-302, miR-372, miR-373, miR-383, and miR-428, siRNA duplexes (siPCAT-1-1, siPCAT-1-2) or NC RNA were transfected into SMMC7721 and Huh7 cells. lncRNA PCAT-1 expression was detected at 48 h after transfection (left, black columns). The transfection efficiency of different miRNAs was confirmed by qRT-PCR (right, gray columns). C and D, inhibition of miR-215 up-regulates PCAT-1 expression. 20 nmol/liter of miR-215 inhibitors and NC RNA were transfected into SMMC7721 and Huh7 HCC cells. All data of PCAT-1 expression were normalized to β-actin mRNA expression levels. All miRNA expression data were normalized to U6 small RNA expression. E, schematic constructions of pGL3-PCAT-1 and pGL3-Mut215. F, pGL3-PCAT-1 and pGL3-Mut215 were co-transfected into SMMC7721 and Huh7 cells with miR-215 mimics or NC RNA. Luciferase activity was detected at 48 h after transfection and normalized relative to the Renilla luciferase expression. Inhibition effects of miR-215 mimics on pGL3-PCAT-1 or pGL3-Mut215 are shown. All results of the mean of triplicate assays with standard deviation are presented. *, p < 0.05; **, p < 0.01.

Article Snippet: All miRNA mimics (miR-15a, miR-16, miR-22, miR-93a, miR-105, miR-106a, miR-129, miR-138, miR-181a, miR-192, miR-205, miR-215, miR-291a, miR-294, miR-295, miR-302, miR-372, miR-373, miR-383, and miR-428), miRNA inhibitors (miR-215), and small interfering RNA (siRNA) duplexes (siPCAT-1-1, siPCAT-1-2, siCRKL-1, and siCRKL-2) were products of Genepharma (Shanghai, China); siPCAT-1-1, 5′-GCAGAAACACCAAUGGAUATT-3′/5′-UAUCCAUUGGUGUUUCUGCTT-3′; siPCAT-1-2, 5′-AUACAUAAGACCAUGGAAATT-3′/5′-UUUCCAUGGUCUUAUGUAUTT-3′; siCRKL-1, 5′-UCUGCUCUACCAUGUUUAATT-3′/5′-UUAAACAUGGUAGAGCAGAGC-3′; siCRKL-2, 5′-UGAAAGUCACAAGGAUGAATT-3′/5′-UUCAUCCUUGUGACUUUCACG-3′.

Techniques: Expressing, Transfection, Quantitative RT-PCR, Inhibition, RNA Expression, Luciferase, Activity Assay, Standard Deviation